《The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage in costal cartilage defects》
Following on September 26th, top international interdisciplinary journals in the field of materials science "Advanced Healthcare Materials" published the full text in Biosis Healing's research results, the good news came again! The global authoritative journal of biomaterial "Acta Biomaterialia" published the full text in joint research results of Biosis Healing and Peking Union Medical College Hospital Plastic Surgery Hospital, titled "The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage in costal cartilage defects". This journal has an impact factor of 8.947, which is the Top journal of the First district of the Chinese Academy of Sciences.
This research was completed by Professor Zhao Bo, chief engineer Wang Hongquan from Biosis Healing, and Professor Jiang Haiyue and the team of Professor Pan Bo from Peking Union Medical College Hospital Plastic Surgery Hospital. Through in vitro material analysis and tissue regeneration research in animals of porcine-derived extracellular cartilage, rigorous animal experiment comparison data show that porcine-derived costal cartilage and auricular cartilage extracellular materials have developed well in the application of costal cartilage defect repair prospect.
It is worth noting that, in this study, the decellularization of porcine-derived costal cartilage and auricular cartilage used the decellularization technology of Biosis Healing with independent intellectual property rights. 【Explanation: Animal-derived materials contain many undesirable ingredients. Implantation in the body without professional treatment will cause severe immune rejection and even lead to life-threatening. However, the professional treatment requirements are extremely high, and traditional strong treatment reagents cannot be used to avoid material strength reduction and breakage, and even reagent residues implanted in the body to cause inflammation. Facing technical difficulties, Biosis Healing organized a research and development team to conduct systematic research on the physical, chemical and biological properties of porcine-derived materials from scratch. After many trials and improvements, it has broken through the material processing and mass production process problems of animal-derived tissue quality control, immunogen removal technology, non-crosslinking molding technology, and biological activity retention technology.】The decellularized porcine cartilage and decellularized porcine small intestinal submucosa processed by Biosis Healing's decellularization technology have lower DNA and α-Gal content.
In this study, in order to apply xenogeneic extracellular cartilage to the defect of costal cartilage, a variety of material analysis tests were carried out on the porcine-derived auricle and costal cartilage. Through the independent intellectual property rights of the decellularity technology of Biosis Healing, part of the DNA and α-Gal are effectively removed, no obvious cell nucleus or structural damage is observed in the extracellular matrix, and the collagen is well preserved after the decellularization process. In the in vivo experiments, both extracellular cartilage groups promoted tissue regeneration in cartilage defect areas, accompanied by significant cartilage morphology and cartilage gene expression increase.
At present, a variety of plastic surgery including rhinoplasty and auricle plastic surgery uses autologous costal cartilage for shaping, and cartilage defects after collecting multiple costal cartilage may cause local chest wall deformities. In our research, the functional components and structure of the extracellular matrix are well preserved after the decellularization process. The decellularized costal cartilage and auricular cartilage have a certain functional biocompatibility. After filling the defect, it promoted tissue regeneration of cartilage defect area. It is a potential material for repairing cartilage defects, and it is expected to be manufactured in factories with the help of a mature process system.
[Periodical Introduction] "Acta Biomaterialia" is a top journal in the first district of the Chinese Academy of Sciences with an impact factor of 8.947. It is an international authoritative journal in the field of biomedicine. The journal mainly publishes various original research reports, reviews and communication manuscripts in the field of biomaterials science in a broad sense, summarizes the new progress of existing research, and points out the direction for future development. The content of the papers published in "Acta Biomaterialia" is also regarded as a certain industry indicator and is highly recognized by the industry.
[Introduction of author]
Peking Union Medical College Hospital Plastic Surgery Hospital, dean
Chief physician/academic leader/doctoral supervisor
Peking Union Medical College Hospital Plastic Surgery Hospital, dean
The team leader undertakes a number of scientific research projects such as the National Natural Science Foundation of China, the Beijing Natural Science Foundation, the innovation project of Peking Union Medical College, and Capital Health development Scientific Research project. As the main force of external ear plastic reconstruction in the Peking Union Medical College Hospital Plastic Surgery Hospital, the team has long been committed to the clinical treatment and basic research of external ear plastic reconstruction. In terms of complex external ear deformities, difficult auricle reconstruction, etc., the team makes our country in the international leading position in this field. Studying tissue engineering repair and disease gene exploration, the team has authoritative and innovative in auricle reconstruction at home and abroad.
Biosis Healing founder, doctor of surgery
Professor-level senior engineer/ associate chief physician/ graduate supervisor/ postdoctoral supervisor
As the main completion and project leader, he has undertaken and completed four projects of National Natural Science Foundation of China, Beijing Natural Science Foundation and many scientific research projects.
Biosis Healing's research and development team led by Zhao Bo has conducted systematic research on the physical, chemical and biological properties of porcine-derived materials from scratch, and established a non-cross-linked extracellular matrix-derived biomaterial (SIS material) platform with independent intellectual property rights. It can provide a comprehensive solution for the regeneration and repair of almost all soft tissues in the whole body, such as neurosurgery, stomatology, orthopedics, abdominal wall surgery, thoracic surgery, and gynecology.